T3559
Sigma
Anti-γ-Tubulin antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
| Synonym: | Anti-γ-Tubulin |
Description
| Immunogen | synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin). |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
Properties
| antibody form | IgG fraction of antiserum |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 48 kDa |
| species reactivity | chicken, human |
| application(s) | immunoblotting: 1:1,000 using cultured A431 whole cell extracts |
| indirect immunofluorescence: 1:5,000 using methanol/acetone-fixed chicken fibroblasts | |
| microarray: suitable | |
| Gene Information | human ... TUBG1(7283), TUBG2(27175) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
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References
| reference | Saberi, A., et al., An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells J. Cell Biol. 178, 257-268, (2007) Abstract |
| Rines, D.R., et al., A role for IkappaB kinase 2 in bipolar spindle assembly Proc. Natl. Acad. Sci. U. S. A. 104, 16940-16945, (2007) Abstract |
